Detailed information of TA system TA110764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1181941..1182668
• Replicon: chromosome
• Accession: NZ_CP031919
• Organism: Escherichia coli O145:NM strain FWSEC0002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: CCU01_RS05950
• Protein ID: WP_000547563.1
• Coordinates: 1181941..1182252 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCU01_RS05955
• Protein ID: WP_000126294.1
• Coordinates: 1182249..1182668 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU01_RS05920	1177086..1178795	+	1710	WP_001288147.1	formate hydrogenlyase subunit HycE	-
CCU01_RS05925	1178805..1179347	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CCU01_RS05930	1179347..1180114	+	768	WP_000067400.1	formate hydrogenlyase subunit HycG	-
CCU01_RS05935	1180111..1180521	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
CCU01_RS05940	1180514..1180981	+	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
CCU01_RS05945	1181024..1181779	+	756	WP_001400787.1	hypothetical protein	-
CCU01_RS05950	1181941..1182252	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
CCU01_RS05955	1182249..1182668	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CCU01_RS05960	1182782..1184206	-	1425	WP_000110310.1	6-phospho-beta-glucosidase AscB	-
CCU01_RS05965	1184215..1185672	-	1458	WP_001107816.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CCU01_RS05970	1185933..1186943	+	1011	WP_032161993.1	DNA-binding transcriptional regulator AscG	-
CCU01_RS05975	1187092..1187619	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T110764 WP_000547563.1 NZ_CP031919:1181941-1182252 [Escherichia coli O145:NM]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T110764 NZ_CP031919:1181941-1182252 [Escherichia coli O145:NM]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT110764 WP_000126294.1 NZ_CP031919:1182249-1182668 [Escherichia coli O145:NM]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT110764 NZ_CP031919:1182249-1182668 [Escherichia coli O145:NM]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22