Detailed information of TA system TA110754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5332790..5333392
• Replicon: chromosome
• Accession: NZ_CP031916
• Organism: Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CCU02_RS27470
• Protein ID: WP_000897305.1
• Coordinates: 5333081..5333392 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCU02_RS27465
• Protein ID: WP_000356397.1
• Coordinates: 5332790..5333080 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU02_RS27440	5327865..5329472	-	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
CCU02_RS27445	5330115..5330324	-	210	Protein_5227	hypothetical protein	-
CCU02_RS27450	5330316..5331296	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
CCU02_RS27455	5331525..5331767	-	243	WP_001086388.1	hypothetical protein	-
CCU02_RS27460	5331986..5332204	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CCU02_RS27465	5332790..5333080	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CCU02_RS27470	5333081..5333392	-	312	WP_000897305.1	hypothetical protein	Toxin
CCU02_RS27475	5333621..5334529	+	909	WP_001162704.1	alpha/beta hydrolase	-
CCU02_RS27480	5334593..5335534	-	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
CCU02_RS27485	5335579..5336016	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CCU02_RS27490	5336013..5336885	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CCU02_RS27495	5336879..5337478	-	600	WP_001295269.1	glucose-1-phosphatase	-
CCU02_RS27500	5337577..5338362	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5330316..5331296	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T110754 WP_000897305.1 NZ_CP031916:c5333392-5333081 [Escherichia coli O45:H2]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T110754 NZ_CP031916:c5333392-5333081 [Escherichia coli O45:H2]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT110754 WP_000356397.1 NZ_CP031916:c5333080-5332790 [Escherichia coli O45:H2]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT110754 NZ_CP031916:c5333080-5332790 [Escherichia coli O45:H2]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7