Detailed information of TA system TA110752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4911222..4912021
• Replicon: chromosome
• Accession: NZ_CP031916
• Organism: Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: CCU02_RS25360
• Protein ID: WP_000347267.1
• Coordinates: 4911557..4912021 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: CCU02_RS25355
• Protein ID: WP_001307405.1
• Coordinates: 4911222..4911557 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU02_RS25340	4907007..4907777	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
CCU02_RS25345	4907793..4909127	-	1335	WP_000599627.1	galactarate/glucarate/glycerate transporter GarP	-
CCU02_RS25350	4909502..4911073	+	1572	WP_001273741.1	galactarate dehydratase	-
CCU02_RS25355	4911222..4911557	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CCU02_RS25360	4911557..4912021	+	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CCU02_RS25365	4912076..4912885	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CCU02_RS25370	4913134..4914414	+	1281	WP_000681952.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CCU02_RS25375	4914437..4914910	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CCU02_RS25380	4914921..4915700	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CCU02_RS25385	4915690..4916568	+	879	WP_001399813.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CCU02_RS25390	4916586..4917020	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4899174..4912021	12847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T110752 WP_000347267.1 NZ_CP031916:4911557-4912021 [Escherichia coli O45:H2]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T110752 NZ_CP031916:4911557-4912021 [Escherichia coli O45:H2]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT110752 WP_001307405.1 NZ_CP031916:4911222-4911557 [Escherichia coli O45:H2]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT110752 NZ_CP031916:4911222-4911557 [Escherichia coli O45:H2]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7