Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3484021..3484523 Replicon chromosome
Accession NZ_CP031916
Organism Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CCU02_RS18290 Protein ID WP_000767829.1
Coordinates 3484021..3484275 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CCU02_RS18295 Protein ID WP_001259255.1
Coordinates 3484272..3484523 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU02_RS18260 3479036..3479263 - 228 WP_000985280.1 sulfurtransferase TusA family protein -
CCU02_RS18265 3479277..3480335 - 1059 WP_000492346.1 transport protein YeeE -
CCU02_RS18270 3480514..3481872 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CCU02_RS18275 3481862..3481924 - 63 WP_010723108.1 membrane protein YoeI -
CCU02_RS18280 3482139..3483068 - 930 WP_000803351.1 LysR family transcriptional regulator -
CCU02_RS18285 3483114..3483938 - 825 WP_000754737.1 SDR family oxidoreductase -
CCU02_RS18290 3484021..3484275 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CCU02_RS18295 3484272..3484523 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CCU02_RS18300 3484806..3484856 + 51 WP_001368802.1 his operon leader peptide -
CCU02_RS18305 3485002..3485901 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CCU02_RS18310 3485907..3487211 + 1305 WP_001298841.1 histidinol dehydrogenase -
CCU02_RS18315 3487208..3488278 + 1071 WP_000108980.1 histidinol-phosphate transaminase -
CCU02_RS18320 3488278..3489345 + 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T110746 WP_000767829.1 NZ_CP031916:c3484275-3484021 [Escherichia coli O45:H2]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T110746 NZ_CP031916:c3484275-3484021 [Escherichia coli O45:H2]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT110746 WP_001259255.1 NZ_CP031916:c3484523-3484272 [Escherichia coli O45:H2]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT110746 NZ_CP031916:c3484523-3484272 [Escherichia coli O45:H2]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References