Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1144440..1144981 Replicon chromosome
Accession NZ_CP031916
Organism Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CCU02_RS05530 Protein ID WP_000615976.1
Coordinates 1144440..1144718 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CCU02_RS05535 Protein ID WP_000729704.1
Coordinates 1144721..1144981 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU02_RS05515 1142022..1142600 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CCU02_RS05520 1142806..1143573 + 768 WP_000333380.1 class II glutamine amidotransferase -
CCU02_RS05525 1143544..1144284 - 741 WP_001225679.1 murein L,D-transpeptidase -
CCU02_RS05530 1144440..1144718 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CCU02_RS05535 1144721..1144981 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CCU02_RS05540 1145167..1145940 + 774 WP_000543899.1 C40 family peptidase -
CCU02_RS05545 1145997..1146353 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CCU02_RS05550 1146346..1146624 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CCU02_RS05555 1146729..1148441 - 1713 Protein_1048 flagellar biosynthesis protein FlhA -
CCU02_RS05560 1148413..1149198 + 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T110727 WP_000615976.1 NZ_CP031916:c1144718-1144440 [Escherichia coli O45:H2]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T110727 NZ_CP031916:c1144718-1144440 [Escherichia coli O45:H2]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT110727 WP_000729704.1 NZ_CP031916:c1144981-1144721 [Escherichia coli O45:H2]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT110727 NZ_CP031916:c1144981-1144721 [Escherichia coli O45:H2]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References