Detailed information of TA system TA110714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4327445..4328139
• Replicon: chromosome
• Accession: NZ_CP031913
• Organism: Escherichia coli O157:H7 strain FWSEC0004

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: CAM50_RS22420
• Protein ID: WP_001263495.1
• Coordinates: 4327445..4327843 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CAM50_RS22425
• Protein ID: WP_000554757.1
• Coordinates: 4327846..4328139 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4323032..4323112	-	81	NuclAT_12	-	-
-	4323032..4323112	-	81	NuclAT_12	-	-
-	4323032..4323112	-	81	NuclAT_12	-	-
-	4323032..4323112	-	81	NuclAT_12	-	-
CAM50_RS22395	4323708..4324166	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CAM50_RS22400	4324427..4325884	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CAM50_RS22405	4325941..4326462	-	522	Protein_4258	peptide chain release factor H	-
CAM50_RS22410	4326461..4326664	-	204	Protein_4259	RNA ligase RtcB family protein	-
CAM50_RS22415	4326983..4327435	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
CAM50_RS22420	4327445..4327843	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CAM50_RS22425	4327846..4328139	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CAM50_RS22430	4328191..4329246	-	1056	WP_001226188.1	DNA polymerase IV	-
CAM50_RS22435	4329317..4330102	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CAM50_RS22440	4330074..4331786	+	1713	Protein_4265	flagellar biosynthesis protein FlhA	-
CAM50_RS22445	4331931..4332428	-	498	Protein_4266	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T110714 WP_001263495.1 NZ_CP031913:c4327843-4327445 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T110714 NZ_CP031913:c4327843-4327445 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT110714 WP_000554757.1 NZ_CP031913:c4328139-4327846 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT110714 NZ_CP031913:c4328139-4327846 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6