Detailed information of TA system TA110687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4925859..4926461
• Replicon: chromosome
• Accession: NZ_CP031912
• Organism: Escherichia coli O111:NM strain FWSEC0005

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CCU03_RS24730
• Protein ID: WP_000897305.1
• Coordinates: 4926150..4926461 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCU03_RS24725
• Protein ID: WP_000356397.1
• Coordinates: 4925859..4926149 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU03_RS24700	4921784..4922686	+	903	WP_000331369.1	formate dehydrogenase O subunit beta	-
CCU03_RS24705	4922683..4923318	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
CCU03_RS24710	4923315..4924244	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CCU03_RS24715	4924575..4924817	-	243	WP_001086388.1	hypothetical protein	-
CCU03_RS24720	4925036..4925254	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CCU03_RS24725	4925859..4926149	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CCU03_RS24730	4926150..4926461	-	312	WP_000897305.1	hypothetical protein	Toxin
CCU03_RS24735	4926690..4927598	+	909	WP_001162704.1	alpha/beta hydrolase	-
CCU03_RS24740	4927662..4928603	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CCU03_RS24745	4928648..4929085	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CCU03_RS24750	4929082..4929954	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CCU03_RS24755	4929948..4930547	-	600	WP_001315111.1	glucose-1-phosphatase	-
CCU03_RS24760	4930646..4931431	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T110687 WP_000897305.1 NZ_CP031912:c4926461-4926150 [Escherichia coli O111:NM]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T110687 NZ_CP031912:c4926461-4926150 [Escherichia coli O111:NM]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT110687 WP_000356397.1 NZ_CP031912:c4926149-4925859 [Escherichia coli O111:NM]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT110687 NZ_CP031912:c4926149-4925859 [Escherichia coli O111:NM]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7