Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2681457..2681959 Replicon chromosome
Accession NZ_CP031912
Organism Escherichia coli O111:NM strain FWSEC0005

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CCU03_RS13230 Protein ID WP_000767829.1
Coordinates 2681705..2681959 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CCU03_RS13225 Protein ID WP_001259255.1
Coordinates 2681457..2681708 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU03_RS13200 2676628..2677695 - 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CCU03_RS13205 2677692..2678771 - 1080 WP_000102292.1 histidinol-phosphate transaminase -
CCU03_RS13210 2678768..2680072 - 1305 WP_001345258.1 histidinol dehydrogenase -
CCU03_RS13215 2680078..2680977 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CCU03_RS13220 2681123..2681173 - 51 WP_001364200.1 his operon leader peptide -
CCU03_RS13225 2681457..2681708 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CCU03_RS13230 2681705..2681959 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CCU03_RS13235 2682042..2682866 + 825 WP_000754755.1 SDR family oxidoreductase -
CCU03_RS13240 2682912..2683841 + 930 WP_000803331.1 LysR family transcriptional regulator -
CCU03_RS13245 2684056..2684118 + 63 WP_010723108.1 membrane protein YoeI -
CCU03_RS13250 2684108..2685466 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CCU03_RS13255 2685645..2686703 + 1059 WP_000492340.1 transport protein YeeE -
CCU03_RS13260 2686717..2686944 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T110672 WP_000767829.1 NZ_CP031912:2681705-2681959 [Escherichia coli O111:NM]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T110672 NZ_CP031912:2681705-2681959 [Escherichia coli O111:NM]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT110672 WP_001259255.1 NZ_CP031912:2681457-2681708 [Escherichia coli O111:NM]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT110672 NZ_CP031912:2681457-2681708 [Escherichia coli O111:NM]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References