Detailed information of TA system TA110661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5195351..5195953
• Replicon: chromosome
• Accession: NZ_CP031910
• Organism: Escherichia coli O121:H19 strain FWSEC0006

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: B9X35_RS26380
• Protein ID: WP_000897305.1
• Coordinates: 5195642..5195953 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B9X35_RS26375
• Protein ID: WP_000356397.1
• Coordinates: 5195351..5195641 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9X35_RS26350	5191296..5192198	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
B9X35_RS26355	5192195..5192830	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
B9X35_RS26360	5192827..5193756	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
B9X35_RS26365	5194086..5194328	-	243	WP_001086388.1	hypothetical protein	-
B9X35_RS26370	5194547..5194765	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
B9X35_RS26375	5195351..5195641	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
B9X35_RS26380	5195642..5195953	-	312	WP_000897305.1	hypothetical protein	Toxin
B9X35_RS26385	5196182..5197089	+	908	Protein_5064	alpha/beta hydrolase	-
B9X35_RS26390	5197257..5198171	-	915	WP_072097029.1	transposase	-
B9X35_RS26395	5198184..5199122	-	939	Protein_5066	hypothetical protein	-
B9X35_RS26400	5199486..5200427	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
B9X35_RS26405	5200472..5200909	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T110661 WP_000897305.1 NZ_CP031910:c5195953-5195642 [Escherichia coli O121:H19]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T110661 NZ_CP031910:c5195953-5195642 [Escherichia coli O121:H19]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT110661 WP_000356397.1 NZ_CP031910:c5195641-5195351 [Escherichia coli O121:H19]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT110661 NZ_CP031910:c5195641-5195351 [Escherichia coli O121:H19]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7