Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4245474..4246015 Replicon chromosome
Accession NZ_CP031910
Organism Escherichia coli O121:H19 strain FWSEC0006

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag B9X35_RS21765 Protein ID WP_000615976.1
Coordinates 4245737..4246015 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A4T9JVH9
Locus tag B9X35_RS21760 Protein ID WP_000729706.1
Coordinates 4245474..4245734 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9X35_RS21735 4241257..4242042 - 786 WP_000207558.1 putative lateral flagellar export/assembly protein LafU -
B9X35_RS21740 4242014..4243726 + 1713 Protein_4184 flagellar biosynthesis protein FlhA -
B9X35_RS21745 4243831..4244109 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
B9X35_RS21750 4244102..4244458 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
B9X35_RS21755 4244515..4245288 - 774 WP_000543899.1 C40 family peptidase -
B9X35_RS21760 4245474..4245734 + 261 WP_000729706.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
B9X35_RS21765 4245737..4246015 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
B9X35_RS21770 4246171..4246911 + 741 WP_001225679.1 murein L,D-transpeptidase -
B9X35_RS21775 4246882..4247649 - 768 WP_000333380.1 class II glutamine amidotransferase -
B9X35_RS21780 4247855..4248433 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T110658 WP_000615976.1 NZ_CP031910:4245737-4246015 [Escherichia coli O121:H19]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T110658 NZ_CP031910:4245737-4246015 [Escherichia coli O121:H19]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9390.79 Da        Isoelectric Point: 5.8129

>AT110658 WP_000729706.1 NZ_CP031910:4245474-4245734 [Escherichia coli O121:H19]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADNL
FDKLGI

Download         Length: 261 bp

>AT110658 NZ_CP031910:4245474-4245734 [Escherichia coli O121:H19]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATAATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
AlphaFold DB A0A4T9JVH9

References