Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2005196..2005698 Replicon chromosome
Accession NZ_CP031910
Organism Escherichia coli O121:H19 strain FWSEC0006

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag B9X35_RS09920 Protein ID WP_000767829.1
Coordinates 2005444..2005698 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag B9X35_RS09915 Protein ID WP_001259255.1
Coordinates 2005196..2005447 (+) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9X35_RS09890 2000374..2001441 - 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
B9X35_RS09895 2001441..2002511 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
B9X35_RS09900 2002508..2003812 - 1305 WP_000009587.1 histidinol dehydrogenase -
B9X35_RS09905 2003818..2004717 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
B9X35_RS09910 2004863..2004913 - 51 WP_001364200.1 his operon leader peptide -
B9X35_RS09915 2005196..2005447 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
B9X35_RS09920 2005444..2005698 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
B9X35_RS09925 2005781..2006605 + 825 WP_000754721.1 SDR family oxidoreductase -
B9X35_RS09930 2006651..2007580 + 930 WP_000803351.1 LysR family transcriptional regulator -
B9X35_RS09935 2007795..2007857 + 63 WP_010723108.1 membrane protein YoeI -
B9X35_RS09940 2007847..2009205 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
B9X35_RS09945 2009384..2010442 + 1059 WP_000492305.1 transport protein YeeE -
B9X35_RS09950 2010456..2010683 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T110644 WP_000767829.1 NZ_CP031910:2005444-2005698 [Escherichia coli O121:H19]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T110644 NZ_CP031910:2005444-2005698 [Escherichia coli O121:H19]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT110644 WP_001259255.1 NZ_CP031910:2005196-2005447 [Escherichia coli O121:H19]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT110644 NZ_CP031910:2005196-2005447 [Escherichia coli O121:H19]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References