Detailed information of TA system TA110642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1177946..1178673
• Replicon: chromosome
• Accession: NZ_CP031910
• Organism: Escherichia coli O121:H19 strain FWSEC0006

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: B9X35_RS05900
• Protein ID: WP_000547564.1
• Coordinates: 1177946..1178257 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B9X35_RS05905
• Protein ID: WP_000126294.1
• Coordinates: 1178254..1178673 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9X35_RS05870	1173088..1174797	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
B9X35_RS05875	1174807..1175349	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
B9X35_RS05880	1175349..1176116	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
B9X35_RS05885	1176113..1176523	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
B9X35_RS05890	1176516..1176986	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
B9X35_RS05895	1177011..1177772	+	762	WP_001026445.1	hypothetical protein	-
B9X35_RS05900	1177946..1178257	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
B9X35_RS05905	1178254..1178673	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
B9X35_RS05910	1178788..1180212	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
B9X35_RS05915	1180221..1181597	-	1377	WP_001107877.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
B9X35_RS05920	1181857..1182867	+	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
B9X35_RS05925	1183016..1183543	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T110642 WP_000547564.1 NZ_CP031910:1177946-1178257 [Escherichia coli O121:H19]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T110642 NZ_CP031910:1177946-1178257 [Escherichia coli O121:H19]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT110642 WP_000126294.1 NZ_CP031910:1178254-1178673 [Escherichia coli O121:H19]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT110642 NZ_CP031910:1178254-1178673 [Escherichia coli O121:H19]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAAAGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5