Detailed information of TA system TA110632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5196658..5197260
• Replicon: chromosome
• Accession: NZ_CP031908
• Organism: Escherichia coli O103:H2 strain FWSEC0007

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CCU04_RS26480
• Protein ID: WP_000897305.1
• Coordinates: 5196949..5197260 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCU04_RS26475
• Protein ID: WP_000356397.1
• Coordinates: 5196658..5196948 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU04_RS26445	5192224..5192859	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CCU04_RS26450	5192856..5193785	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CCU04_RS26460	5194184..5195164	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
CCU04_RS26465	5195393..5195635	-	243	WP_001086388.1	hypothetical protein	-
CCU04_RS26470	5195854..5196072	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CCU04_RS26475	5196658..5196948	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CCU04_RS26480	5196949..5197260	-	312	WP_000897305.1	hypothetical protein	Toxin
CCU04_RS26485	5197489..5198397	+	909	WP_001162704.1	alpha/beta hydrolase	-
CCU04_RS26490	5198461..5199402	-	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
CCU04_RS26495	5199447..5199884	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CCU04_RS26500	5199881..5200753	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CCU04_RS26505	5200747..5201346	-	600	WP_001295269.1	glucose-1-phosphatase	-
CCU04_RS26510	5201445..5202230	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5194184..5195164	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T110632 WP_000897305.1 NZ_CP031908:c5197260-5196949 [Escherichia coli O103:H2]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T110632 NZ_CP031908:c5197260-5196949 [Escherichia coli O103:H2]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT110632 WP_000356397.1 NZ_CP031908:c5196948-5196658 [Escherichia coli O103:H2]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT110632 NZ_CP031908:c5196948-5196658 [Escherichia coli O103:H2]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7