Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4289263..4289804 Replicon chromosome
Accession NZ_CP031908
Organism Escherichia coli O103:H2 strain FWSEC0007

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CCU04_RS22210 Protein ID WP_000615976.1
Coordinates 4289526..4289804 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CCU04_RS22205 Protein ID WP_000729704.1
Coordinates 4289263..4289523 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU04_RS22180 4285046..4285831 - 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -
CCU04_RS22185 4285803..4287515 + 1713 Protein_4249 flagellar biosynthesis protein FlhA -
CCU04_RS22190 4287620..4287898 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CCU04_RS22195 4287891..4288247 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CCU04_RS22200 4288304..4289077 - 774 WP_000543899.1 C40 family peptidase -
CCU04_RS22205 4289263..4289523 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CCU04_RS22210 4289526..4289804 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CCU04_RS22215 4289960..4290700 + 741 WP_001225679.1 murein L,D-transpeptidase -
CCU04_RS22220 4290671..4291438 - 768 WP_000333380.1 class II glutamine amidotransferase -
CCU04_RS22225 4291644..4292222 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T110627 WP_000615976.1 NZ_CP031908:4289526-4289804 [Escherichia coli O103:H2]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T110627 NZ_CP031908:4289526-4289804 [Escherichia coli O103:H2]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT110627 WP_000729704.1 NZ_CP031908:4289263-4289523 [Escherichia coli O103:H2]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT110627 NZ_CP031908:4289263-4289523 [Escherichia coli O103:H2]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References