Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1956871..1957373 Replicon chromosome
Accession NZ_CP031908
Organism Escherichia coli O103:H2 strain FWSEC0007

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CCU04_RS09555 Protein ID WP_000767829.1
Coordinates 1957119..1957373 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CCU04_RS09550 Protein ID WP_001259255.1
Coordinates 1956871..1957122 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU04_RS09525 1952049..1953116 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CCU04_RS09530 1953116..1954186 - 1071 WP_000108980.1 histidinol-phosphate transaminase -
CCU04_RS09535 1954183..1955487 - 1305 WP_001298841.1 histidinol dehydrogenase -
CCU04_RS09540 1955493..1956392 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CCU04_RS09545 1956538..1956588 - 51 WP_001368802.1 his operon leader peptide -
CCU04_RS09550 1956871..1957122 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CCU04_RS09555 1957119..1957373 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CCU04_RS09560 1957456..1958280 + 825 WP_000754737.1 SDR family oxidoreductase -
CCU04_RS09565 1958326..1959255 + 930 WP_000803351.1 LysR family transcriptional regulator -
CCU04_RS09570 1959470..1959532 + 63 WP_010723108.1 membrane protein YoeI -
CCU04_RS09575 1959522..1960880 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CCU04_RS09580 1961059..1962117 + 1059 WP_000492346.1 transport protein YeeE -
CCU04_RS09585 1962131..1962358 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T110612 WP_000767829.1 NZ_CP031908:1957119-1957373 [Escherichia coli O103:H2]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T110612 NZ_CP031908:1957119-1957373 [Escherichia coli O103:H2]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT110612 WP_001259255.1 NZ_CP031908:1956871-1957122 [Escherichia coli O103:H2]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT110612 NZ_CP031908:1956871-1957122 [Escherichia coli O103:H2]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References