Detailed information of TA system TA110601

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19768..20022
• Replicon: plasmid unnamed17
• Accession: NZ_CP031907
• Organism: Escherichia coli O91:H21 strain FWSEC0008

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CAX06_RS24655
• Protein ID: WP_032215358.1
• Coordinates: 19816..20022 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	19768..19829 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAX06_RS24620	15219..15959	+	741	WP_000206160.1	conjugal transfer pilus acetylase TraX	-
CAX06_RS24625	16018..16878	+	861	WP_000868985.1	alpha/beta hydrolase	-
CAX06_RS24630	16981..17544	+	564	WP_000139382.1	fertility inhibition protein FinO	-
CAX06_RS24635	17698..17901	+	204	WP_001327131.1	hypothetical protein	-
CAX06_RS24640	18646..19107	+	462	WP_000417388.1	thermonuclease family protein	-
CAX06_RS24645	19210..19593	+	384	WP_001109066.1	hypothetical protein	-
-	19768..19829	-	62	NuclAT_0	-	Antitoxin
-	19768..19829	-	62	NuclAT_0	-	Antitoxin
-	19768..19829	-	62	NuclAT_0	-	Antitoxin
-	19768..19829	-	62	NuclAT_0	-	Antitoxin
CAX06_RS24655	19816..20022	+	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
CAX06_RS24660	20306..20554	+	249	WP_000083842.1	replication regulatory protein RepA	-
CAX06_RS24670	20800..20874	+	75	WP_001367777.1	RepA leader peptide Tap	-
CAX06_RS24675	20867..21724	+	858	WP_000130938.1	incFII family plasmid replication initiator RepA	-
CAX06_RS24690	22697..22936	+	240	WP_000859018.1	MarR family transcriptional regulator	-
CAX06_RS24695	23279..23694	-	416	Protein_24	transposase	-
CAX06_RS24700	23977..24399	+	423	WP_001212724.1	Exc2 family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD	1..121036	121036
	-	flank	IS/Tn	-	-	23267..23581	314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T110601 WP_032215358.1 NZ_CP031907:19816-20022 [Escherichia coli O91:H21]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T110601 NZ_CP031907:19816-20022 [Escherichia coli O91:H21]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT110601 NZ_CP031907:c19829-19768 [Escherichia coli O91:H21]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin