Detailed information of TA system TA110599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4732126..4732728
• Replicon: chromosome
• Accession: NZ_CP031906
• Organism: Escherichia coli O91:H21 strain FWSEC0008

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CAX06_RS23275
• Protein ID: WP_000897305.1
• Coordinates: 4732417..4732728 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CAX06_RS23270
• Protein ID: WP_000356397.1
• Coordinates: 4732126..4732416 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAX06_RS23245	4728071..4728973	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CAX06_RS23250	4728970..4729605	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CAX06_RS23255	4729602..4730531	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CAX06_RS23260	4730861..4731103	-	243	WP_001087409.1	hypothetical protein	-
CAX06_RS23265	4731322..4731540	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CAX06_RS23270	4732126..4732416	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CAX06_RS23275	4732417..4732728	-	312	WP_000897305.1	hypothetical protein	Toxin
CAX06_RS23280	4732957..4733655	+	699	Protein_4471	alpha/beta hydrolase	-
CAX06_RS23285	4733928..4734512	-	585	WP_001056416.1	hypothetical protein	-
CAX06_RS23290	4734680..4734928	+	249	WP_001310454.1	helix-turn-helix domain-containing protein	-
CAX06_RS23295	4734930..4737020	+	2091	WP_096958021.1	Mu transposase C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T110599 WP_000897305.1 NZ_CP031906:c4732728-4732417 [Escherichia coli O91:H21]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T110599 NZ_CP031906:c4732728-4732417 [Escherichia coli O91:H21]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT110599 WP_000356397.1 NZ_CP031906:c4732416-4732126 [Escherichia coli O91:H21]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT110599 NZ_CP031906:c4732416-4732126 [Escherichia coli O91:H21]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7