Detailed information of TA system TA110574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5075407..5076009
• Replicon: chromosome
• Accession: NZ_CP031902
• Organism: Escherichia coli O104:H4 strain FWSEC0009

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C8200_RS25415
• Protein ID: WP_000897305.1
• Coordinates: 5075698..5076009 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C8200_RS25410
• Protein ID: WP_000356397.1
• Coordinates: 5075407..5075697 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C8200_RS25385	5071351..5072253	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
C8200_RS25390	5072250..5072885	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C8200_RS25395	5072882..5073811	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C8200_RS25400	5074141..5074383	-	243	WP_001087409.1	hypothetical protein	-
C8200_RS25405	5074603..5074821	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
C8200_RS25410	5075407..5075697	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C8200_RS25415	5075698..5076009	-	312	WP_000897305.1	hypothetical protein	Toxin
C8200_RS25420	5076238..5077146	+	909	WP_001387201.1	alpha/beta hydrolase	-
C8200_RS25425	5077210..5078151	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C8200_RS25430	5078196..5078633	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C8200_RS25435	5078630..5079502	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C8200_RS25440	5079496..5080095	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T110574 WP_000897305.1 NZ_CP031902:c5076009-5075698 [Escherichia coli O104:H4]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T110574 NZ_CP031902:c5076009-5075698 [Escherichia coli O104:H4]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT110574 WP_000356397.1 NZ_CP031902:c5075697-5075407 [Escherichia coli O104:H4]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT110574 NZ_CP031902:c5075697-5075407 [Escherichia coli O104:H4]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7