Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4176584..4177125 Replicon chromosome
Accession NZ_CP031902
Organism Escherichia coli O104:H4 strain FWSEC0009

Toxin (Protein)


Gene name yafQ Uniprot ID B7LHF6
Locus tag C8200_RS21125 Protein ID WP_000615974.1
Coordinates 4176847..4177125 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C8200_RS21120 Protein ID WP_000729704.1
Coordinates 4176584..4176844 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8200_RS21095 4172367..4173152 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
C8200_RS21100 4173124..4174836 + 1713 Protein_4034 flagellar biosynthesis protein FlhA -
C8200_RS21105 4174941..4175219 + 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
C8200_RS21110 4175212..4175568 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C8200_RS21115 4175625..4176398 - 774 WP_000543895.1 C40 family peptidase -
C8200_RS21120 4176584..4176844 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C8200_RS21125 4176847..4177125 + 279 WP_000615974.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C8200_RS21130 4177281..4178021 + 741 WP_001225679.1 murein L,D-transpeptidase -
C8200_RS21135 4177992..4178759 - 768 WP_000333380.1 class II glutamine amidotransferase -
C8200_RS21140 4178965..4179543 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10855.68 Da        Isoelectric Point: 10.2267

>T110570 WP_000615974.1 NZ_CP031902:4176847-4177125 [Escherichia coli O104:H4]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMRLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T110570 NZ_CP031902:4176847-4177125 [Escherichia coli O104:H4]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGAGGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT110570 WP_000729704.1 NZ_CP031902:4176584-4176844 [Escherichia coli O104:H4]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT110570 NZ_CP031902:4176584-4176844 [Escherichia coli O104:H4]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y514

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References