Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1968737..1969239 Replicon chromosome
Accession NZ_CP031902
Organism Escherichia coli O104:H4 strain FWSEC0009

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C8200_RS09670 Protein ID WP_000767829.1
Coordinates 1968985..1969239 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C8200_RS09665 Protein ID WP_001259255.1
Coordinates 1968737..1968988 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8200_RS09640 1963914..1964981 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C8200_RS09645 1964981..1966051 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
C8200_RS09650 1966048..1967352 - 1305 WP_001386882.1 histidinol dehydrogenase -
C8200_RS09655 1967358..1968257 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C8200_RS09660 1968403..1968453 - 51 WP_001364200.1 his operon leader peptide -
C8200_RS09665 1968737..1968988 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C8200_RS09670 1968985..1969239 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C8200_RS09675 1969322..1970146 + 825 WP_000754758.1 SDR family oxidoreductase -
C8200_RS09680 1970192..1971121 + 930 WP_000803366.1 LysR family transcriptional regulator -
C8200_RS09685 1971336..1971398 + 63 WP_010723108.1 membrane protein YoeI -
C8200_RS09690 1971388..1972746 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C8200_RS09695 1972925..1973983 + 1059 WP_000492324.1 transport protein YeeE -
C8200_RS09700 1973997..1974224 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T110557 WP_000767829.1 NZ_CP031902:1968985-1969239 [Escherichia coli O104:H4]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T110557 NZ_CP031902:1968985-1969239 [Escherichia coli O104:H4]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT110557 WP_001259255.1 NZ_CP031902:1968737-1968988 [Escherichia coli O104:H4]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT110557 NZ_CP031902:1968737-1968988 [Escherichia coli O104:H4]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References