Detailed information of TA system TA110548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4752308..4752910
• Replicon: chromosome
• Accession: NZ_CP031898
• Organism: Escherichia coli O113:H21 strain FWSEC0010

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C8201_RS23405
• Protein ID: WP_000897305.1
• Coordinates: 4752599..4752910 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C8201_RS23400
• Protein ID: WP_000356397.1
• Coordinates: 4752308..4752598 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C8201_RS23375	4748234..4749136	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
C8201_RS23380	4749133..4749768	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C8201_RS23385	4749765..4750694	+	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
C8201_RS23390	4751024..4751266	-	243	WP_001087409.1	hypothetical protein	-
C8201_RS23395	4751485..4751703	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C8201_RS23400	4752308..4752598	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C8201_RS23405	4752599..4752910	-	312	WP_000897305.1	hypothetical protein	Toxin
C8201_RS23410	4753139..4754047	+	909	WP_001162704.1	alpha/beta hydrolase	-
C8201_RS23415	4754111..4755052	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C8201_RS23420	4755097..4755534	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C8201_RS23425	4755531..4756403	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C8201_RS23430	4756397..4756996	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T110548 WP_000897305.1 NZ_CP031898:c4752910-4752599 [Escherichia coli O113:H21]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T110548 NZ_CP031898:c4752910-4752599 [Escherichia coli O113:H21]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT110548 WP_000356397.1 NZ_CP031898:c4752598-4752308 [Escherichia coli O113:H21]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT110548 NZ_CP031898:c4752598-4752308 [Escherichia coli O113:H21]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7