Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3892782..3893323 Replicon chromosome
Accession NZ_CP031898
Organism Escherichia coli O113:H21 strain FWSEC0010

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag C8201_RS19390 Protein ID WP_000615976.1
Coordinates 3893045..3893323 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C8201_RS19385 Protein ID WP_000729704.1
Coordinates 3892782..3893042 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8201_RS19360 3888565..3889350 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
C8201_RS19365 3889295..3891034 + 1740 WP_001296890.1 flagellar biosynthesis protein FlhA -
C8201_RS19370 3891139..3891417 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C8201_RS19375 3891410..3891766 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
C8201_RS19380 3891823..3892596 - 774 WP_000543895.1 C40 family peptidase -
C8201_RS19385 3892782..3893042 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C8201_RS19390 3893045..3893323 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C8201_RS19395 3893479..3894219 + 741 WP_001225679.1 murein L,D-transpeptidase -
C8201_RS19400 3894190..3894957 - 768 WP_000333380.1 class II glutamine amidotransferase -
C8201_RS19405 3895163..3895741 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3891410..3904109 12699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T110545 WP_000615976.1 NZ_CP031898:3893045-3893323 [Escherichia coli O113:H21]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T110545 NZ_CP031898:3893045-3893323 [Escherichia coli O113:H21]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT110545 WP_000729704.1 NZ_CP031898:3892782-3893042 [Escherichia coli O113:H21]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT110545 NZ_CP031898:3892782-3893042 [Escherichia coli O113:H21]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References