Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1894168..1894670 Replicon chromosome
Accession NZ_CP031898
Organism Escherichia coli O113:H21 strain FWSEC0010

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C8201_RS09180 Protein ID WP_000767829.1
Coordinates 1894416..1894670 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C8201_RS09175 Protein ID WP_001259255.1
Coordinates 1894168..1894419 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8201_RS09150 1889346..1890413 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C8201_RS09155 1890413..1891483 - 1071 WP_000108962.1 histidinol-phosphate transaminase -
C8201_RS09160 1891480..1892784 - 1305 WP_001369878.1 histidinol dehydrogenase -
C8201_RS09165 1892790..1893689 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C8201_RS09170 1893835..1893885 - 51 WP_001364200.1 his operon leader peptide -
C8201_RS09175 1894168..1894419 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C8201_RS09180 1894416..1894670 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C8201_RS09185 1894753..1895577 + 825 WP_000754749.1 SDR family oxidoreductase -
C8201_RS09190 1895623..1896552 + 930 WP_000803351.1 LysR family transcriptional regulator -
C8201_RS09195 1896767..1896829 + 63 WP_010723108.1 membrane protein YoeI -
C8201_RS09200 1896819..1898177 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C8201_RS09205 1898352..1899408 + 1057 Protein_1764 transport protein YeeE -
C8201_RS09210 1899422..1899649 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T110532 WP_000767829.1 NZ_CP031898:1894416-1894670 [Escherichia coli O113:H21]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T110532 NZ_CP031898:1894416-1894670 [Escherichia coli O113:H21]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT110532 WP_001259255.1 NZ_CP031898:1894168-1894419 [Escherichia coli O113:H21]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT110532 NZ_CP031898:1894168-1894419 [Escherichia coli O113:H21]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References