Detailed information of TA system TA110373

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69401..69654
• Replicon: plasmid pKP121-3
• Accession: NZ_CP031852
• Organism: Klebsiella pneumoniae strain 121

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: D1Y71_RS28555
• Protein ID: WP_001312851.1
• Coordinates: 69505..69654 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	69401..69460 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1Y71_RS28510	64579..65040	+	462	WP_001233838.1	thermonuclease family protein	-
D1Y71_RS28515	65086..65295	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
D1Y71_RS28520	65333..65923	+	591	WP_033807967.1	DUF2726 domain-containing protein	-
D1Y71_RS28525	66110..67681	-	1572	WP_001526014.1	IS66 family transposase	-
D1Y71_RS28530	67701..68048	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
D1Y71_RS28535	68048..68725	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
D1Y71_RS28545	68785..69258	+	474	WP_016240489.1	hypothetical protein	-
-	69401..69460	-	60	NuclAT_1	-	Antitoxin
-	69401..69460	-	60	NuclAT_1	-	Antitoxin
-	69401..69460	-	60	NuclAT_1	-	Antitoxin
-	69401..69460	-	60	NuclAT_1	-	Antitoxin
D1Y71_RS28555	69505..69654	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
D1Y71_RS28560	69939..70187	+	249	WP_000083839.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..70498	70498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T110373 WP_001312851.1 NZ_CP031852:69505-69654 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T110373 NZ_CP031852:69505-69654 [Klebsiella pneumoniae]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT110373 NZ_CP031852:c69460-69401 [Klebsiella pneumoniae]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin