Detailed information of TA system TA110370

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25368..25637
• Replicon: plasmid pKP121-3
• Accession: NZ_CP031852
• Organism: Klebsiella pneumoniae strain 121

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: D1Y71_RS28275
• Protein ID: WP_001312861.1
• Coordinates: 25479..25637 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	25368..25433 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1Y71_RS28250	21137..21664	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
D1Y71_RS28255	21722..21955	+	234	WP_000006003.1	DUF905 family protein	-
D1Y71_RS28260	22016..23980	+	1965	WP_025746229.1	ParB/RepB/Spo0J family partition protein	-
D1Y71_RS28265	24049..24483	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
D1Y71_RS28270	24480..25199	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	25211..25435	+	225	NuclAT_0	-	-
-	25211..25435	+	225	NuclAT_0	-	-
-	25211..25435	+	225	NuclAT_0	-	-
-	25211..25435	+	225	NuclAT_0	-	-
-	25368..25433	+	66	-	-	Antitoxin
D1Y71_RS28275	25479..25637	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
D1Y71_RS28280	25938..26142	-	205	Protein_34	pilus protein	-
D1Y71_RS28290	26534..26830	+	297	WP_001272251.1	hypothetical protein	-
D1Y71_RS28295	26941..27762	+	822	WP_001234445.1	DUF945 domain-containing protein	-
D1Y71_RS28300	28059..28649	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
D1Y71_RS28305	28982..29365	+	384	WP_001151529.1	relaxosome protein TraM	-
D1Y71_RS28310	29558..30205	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
D1Y71_RS28315	30313..30552	+	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..70498	70498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T110370 WP_001312861.1 NZ_CP031852:25479-25637 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T110370 NZ_CP031852:25479-25637 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT110370 NZ_CP031852:25368-25433 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin