Detailed information of TA system TA110317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1441729..1442328
• Replicon: chromosome
• Accession: NZ_CP031843
• Organism: Bartonella kosoyi strain Tel Aviv

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5B9CYQ5
• Locus tag: D1093_RS06210
• Protein ID: WP_012230946.1
• Coordinates: 1441729..1442010 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D1093_RS06215
• Protein ID: WP_120101387.1
• Coordinates: 1442029..1442328 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1093_RS06185	1438830..1439009	+	180	WP_150222287.1	hypothetical protein	-
D1093_RS06190	1439021..1439467	+	447	WP_120102364.1	phage tail protein	-
D1093_RS06195	1439464..1439718	+	255	WP_120101381.1	tail protein X	-
D1093_RS06200	1439709..1440719	+	1011	WP_150222289.1	late control protein	-
D1093_RS06205	1440793..1440867	+	75	Protein_1183	chromosome partitioning protein ParB	-
D1093_RS06210	1441729..1442010	+	282	WP_012230946.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1093_RS06215	1442029..1442328	+	300	WP_120101387.1	HigA family addiction module antidote protein	Antitoxin
D1093_RS06220	1442385..1443422	-	1038	WP_120101389.1	tyrosine-type recombinase/integrase	-
D1093_RS06225	1443425..1443673	-	249	WP_100129737.1	hypothetical protein	-
D1093_RS06230	1443785..1444408	-	624	WP_120101391.1	YqaJ viral recombinase family protein	-
D1093_RS06235	1444410..1445186	-	777	WP_120101393.1	ERF family protein	-
D1093_RS06240	1445263..1445634	-	372	WP_120101395.1	hypothetical protein	-
D1093_RS06245	1445638..1446108	-	471	WP_120101397.1	hypothetical protein	-
D1093_RS06250	1446102..1446503	-	402	WP_120101399.1	hypothetical protein	-
D1093_RS06255	1446503..1447042	-	540	WP_120101400.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1436513..1475285	38772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-56)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10675.46 Da
• Isoelectric Point: 10.2972

>T110317 WP_012230946.1 NZ_CP031843:1441729-1442010 [Bartonella kosoyi]
MAIVSFKHKGLKLFYTTGSTKAIRPDHVKKLRVILTALTSATTPDMLRAPAFKMHPLKGELTGYYSIWVNGNWRVTFRFI
GTDVELVDYQDYH

Nucleotide

• Length: 282 bp

>T110317 NZ_CP031843:1441729-1442010 [Bartonella kosoyi]
ATGGCAATTGTGAGTTTCAAACATAAGGGCTTAAAATTATTTTATACAACAGGTTCCACAAAAGCTATTCGACCAGATCA
CGTAAAGAAATTACGCGTTATCTTAACGGCTTTAACAAGTGCTACGACACCCGATATGTTGCGAGCACCTGCCTTTAAAA
TGCATCCTCTTAAAGGTGAACTTACAGGATATTATTCTATATGGGTGAATGGAAATTGGCGTGTTACTTTTCGCTTTATA
GGAACAGATGTGGAGCTTGTTGATTATCAAGATTACCATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11140.94 Da
• Isoelectric Point: 6.9876

>AT110317 WP_120101387.1 NZ_CP031843:1442029-1442328 [Bartonella kosoyi]
MMHNPAHPGEVLKVAFLEEMGITIQKLADHLHMTRASLSRVINGHASMSTELAIKLELAGFSKAKFWLDMQTNYNLWQTM
QQTQPPISPLVSDVPQTQL

Nucleotide

• Length: 300 bp

>AT110317 NZ_CP031843:1442029-1442328 [Bartonella kosoyi]
ATGATGCATAACCCCGCACATCCGGGCGAAGTTCTAAAAGTAGCTTTCTTAGAAGAAATGGGGATAACGATACAAAAGTT
AGCGGATCATCTCCATATGACAAGAGCTTCTCTATCAAGAGTGATTAACGGTCATGCTTCTATGAGTACCGAACTTGCAA
TTAAATTAGAATTAGCTGGTTTTAGTAAAGCAAAATTTTGGTTGGATATGCAAACAAACTATAATTTATGGCAAACAATG
CAACAGACACAACCACCTATTTCTCCTCTGGTTTCTGATGTACCTCAAACGCAGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A5B9CYQ5