Detailed information of TA system TA110266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3740609..3741303
• Replicon: chromosome
• Accession: NZ_CP031833
• Organism: Escherichia coli strain 2452

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: D0R13_RS19310
• Protein ID: WP_001263493.1
• Coordinates: 3740609..3741007 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: D0R13_RS19315
• Protein ID: WP_032162515.1
• Coordinates: 3741010..3741303 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0R13_RS19280	3735609..3736853	-	1245	WP_000189541.1	esterase FrsA	-
-	3736269..3736349	-	81	NuclAT_10	-	-
-	3736269..3736349	-	81	NuclAT_10	-	-
-	3736269..3736349	-	81	NuclAT_10	-	-
-	3736269..3736349	-	81	NuclAT_10	-	-
D0R13_RS19285	3736945..3737403	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
D0R13_RS19290	3737664..3739121	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
D0R13_RS19295	3739178..3739699	-	522	Protein_3527	peptide chain release factor H	-
D0R13_RS19300	3739698..3739901	-	204	Protein_3528	RNA ligase RtcB family protein	-
D0R13_RS19305	3740147..3740599	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
D0R13_RS19310	3740609..3741007	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
D0R13_RS19315	3741010..3741303	-	294	WP_032162515.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
D0R13_RS19320	3741355..3742410	-	1056	WP_001226164.1	DNA polymerase IV	-
D0R13_RS19325	3742481..3743266	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
D0R13_RS19330	3743238..3744950	+	1713	Protein_3534	flagellar biosynthesis protein FlhA	-
D0R13_RS19335	3745174..3745671	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T110266 WP_001263493.1 NZ_CP031833:c3741007-3740609 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T110266 NZ_CP031833:c3741007-3740609 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11261.86 Da
• Isoelectric Point: 5.9050

>AT110266 WP_032162515.1 NZ_CP031833:c3741303-3741010 [Escherichia coli]
MHRILAEKSVNITELRKNPVKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT110266 NZ_CP031833:c3741303-3741010 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGTTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8