Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1835556..1836058 Replicon chromosome
Accession NZ_CP031833
Organism Escherichia coli strain 2452

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag D0R13_RS09280 Protein ID WP_000767829.1
Coordinates 1835804..1836058 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag D0R13_RS09275 Protein ID WP_001259255.1
Coordinates 1835556..1835807 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0R13_RS09245 1830733..1831800 - 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
D0R13_RS09250 1831800..1832870 - 1071 WP_094319327.1 histidinol-phosphate transaminase -
D0R13_RS09255 1832867..1834171 - 1305 WP_001396164.1 histidinol dehydrogenase -
D0R13_RS09260 1834177..1835076 - 900 WP_000566782.1 ATP phosphoribosyltransferase -
D0R13_RS09270 1835222..1835272 - 51 WP_001364200.1 his operon leader peptide -
D0R13_RS09275 1835556..1835807 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
D0R13_RS09280 1835804..1836058 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
D0R13_RS09285 1836141..1836965 + 825 WP_000754754.1 SDR family oxidoreductase -
D0R13_RS09290 1837011..1837940 + 930 WP_000803351.1 LysR family transcriptional regulator -
D0R13_RS09295 1838155..1838217 + 63 WP_010723108.1 membrane protein YoeI -
D0R13_RS09300 1838207..1839565 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
D0R13_RS09305 1839744..1840802 + 1059 WP_000492305.1 transport protein YeeE -
D0R13_RS09310 1840816..1841043 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T110253 WP_000767829.1 NZ_CP031833:1835804-1836058 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T110253 NZ_CP031833:1835804-1836058 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT110253 WP_001259255.1 NZ_CP031833:1835556-1835807 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT110253 NZ_CP031833:1835556-1835807 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References