Detailed information of TA system TA110236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3711187..3711784
• Replicon: chromosome
• Accession: NZ_CP031817
• Organism: Klebsiella pneumoniae strain INF235-sc-2280127

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: D0898_RS18505
• Protein ID: WP_004893639.1
• Coordinates: 3711467..3711784 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: D0898_RS18500
• Protein ID: WP_004142561.1
• Coordinates: 3711187..3711474 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0898_RS18475	3706457..3707572	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
D0898_RS18480	3707752..3708333	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
D0898_RS18485	3708333..3708701	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
D0898_RS18490	3708821..3709474	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
D0898_RS18500	3711187..3711474	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
D0898_RS18505	3711467..3711784	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D0898_RS18510	3711969..3713012	-	1044	WP_009308098.1	DUF2157 domain-containing protein	-
D0898_RS18520	3713678..3714544	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
D0898_RS18525	3714653..3716080	+	1428	WP_009308097.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T110236 WP_004893639.1 NZ_CP031817:c3711784-3711467 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T110236 NZ_CP031817:c3711784-3711467 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT110236 WP_004142561.1 NZ_CP031817:c3711474-3711187 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT110236 NZ_CP031817:c3711474-3711187 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1