Detailed information of TA system TA110200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 34133..34860
• Replicon: plasmid unnamed
• Accession: NZ_CP031809
• Organism: Klebsiella pneumoniae strain INF206-sc-2280074

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: D0896_RS27125
• Protein ID: WP_011251285.1
• Coordinates: 34133..34444 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D0896_RS27130
• Protein ID: WP_011251286.1
• Coordinates: 34441..34860 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0896_RS27100	30889..31068	-	180	Protein_25	IS5/IS1182 family transposase	-
D0896_RS27105	31108..32646	-	1539	WP_117362302.1	IS66 family transposase	-
D0896_RS27110	32695..33042	-	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
D0896_RS27115	33039..33377	-	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
D0896_RS27120	33470..33928	+	459	Protein_29	DDE-type integrase/transposase/recombinase	-
D0896_RS27125	34133..34444	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
D0896_RS27130	34441..34860	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
D0896_RS27140	35007..35975	+	969	WP_162889634.1	IS5 family transposase	-
D0896_RS27145	36429..37337	+	909	WP_001372261.1	HNH endonuclease	-
D0896_RS27150	37725..38075	-	351	WP_023328274.1	DUF305 domain-containing protein	-
D0896_RS27155	38219..38650	-	432	WP_000790483.1	silver-binding protein SilE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..233468	233468
	-	inside	IScluster/Tn	-	-	30889..35975	5086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T110200 WP_011251285.1 NZ_CP031809:34133-34444 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T110200 NZ_CP031809:34133-34444 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTT
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT110200 WP_011251286.1 NZ_CP031809:34441-34860 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT110200 NZ_CP031809:34441-34860 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCGCTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1