Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3849815..3850412 Replicon chromosome
Accession NZ_CP031798
Organism Klebsiella pneumoniae strain QMP B2-170

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag D0900_RS19400 Protein ID WP_004142563.1
Coordinates 3850095..3850412 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag D0900_RS19395 Protein ID WP_004142561.1
Coordinates 3849815..3850102 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0900_RS19370 3845086..3846201 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
D0900_RS19375 3846380..3846961 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
D0900_RS19380 3846961..3847329 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
D0900_RS19385 3847449..3848102 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
D0900_RS19390 3848288..3849651 + 1364 WP_085955203.1 IS3-like element ISKpn1 family transposase -
D0900_RS19395 3849815..3850102 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
D0900_RS19400 3850095..3850412 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D0900_RS19405 3850597..3851640 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
D0900_RS19415 3852307..3853173 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
D0900_RS19420 3853282..3854709 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T110152 WP_004142563.1 NZ_CP031798:c3850412-3850095 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T110152 NZ_CP031798:c3850412-3850095 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT110152 WP_004142561.1 NZ_CP031798:c3850102-3849815 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT110152 NZ_CP031798:c3850102-3849815 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References