Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 351531..352177 Replicon chromosome
Accession NZ_CP031792
Organism Klebsiella pneumoniae strain INF116-sc-2279924

Toxin (Protein)


Gene name higB Uniprot ID A0A486RBU5
Locus tag D0883_RS01660 Protein ID WP_032410616.1
Coordinates 351531..351878 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag D0883_RS01665 Protein ID WP_002920557.1
Coordinates 351878..352177 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0883_RS01650 347458..348891 + 1434 WP_002920564.1 glycogen synthase GlgA -
D0883_RS01655 348909..351356 + 2448 WP_002920561.1 glycogen phosphorylase -
D0883_RS01660 351531..351878 + 348 WP_032410616.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D0883_RS01665 351878..352177 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
D0883_RS01670 352240..353748 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
D0883_RS01675 353953..354282 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
D0883_RS01680 354333..355163 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
D0883_RS01685 355213..355971 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13545.55 Da        Isoelectric Point: 5.6802

>T110101 WP_032410616.1 NZ_CP031792:351531-351878 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATILILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFEPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T110101 NZ_CP031792:351531-351878 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATTCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGAGCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT110101 WP_002920557.1 NZ_CP031792:351878-352177 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT110101 NZ_CP031792:351878-352177 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486RBU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References