Detailed information of TA system TA110077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 12883..13399
• Replicon: plasmid pLP10266
• Accession: NZ_CP031786
• Organism: Lacticaseibacillus paracasei strain 10266

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A243Q0H7
• Locus tag: D0638_RS15530
• Protein ID: WP_003573774.1
• Coordinates: 12883..13164 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: D0638_RS15535
• Protein ID: WP_016363783.1
• Coordinates: 13157..13399 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0638_RS15505	8295..9833	+	1539	WP_149350361.1	oligosaccharide flippase family protein	-
D0638_RS15510	10031..11526	+	1496	Protein_9	IS5 family transposase	-
D0638_RS15515	11662..11844	-	183	Protein_10	recombinase family protein	-
D0638_RS15525	12669..12746	-	78	Protein_12	recombinase family protein	-
D0638_RS15530	12883..13164	-	282	WP_003573774.1	type II toxin-antitoxin system YafQ family toxin	Toxin
D0638_RS15535	13157..13399	-	243	WP_016363783.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
D0638_RS15540	13526..13894	-	369	WP_003582572.1	YxeA family protein	-
D0638_RS15545	13929..14849	-	921	WP_149350363.1	IS30 family transposase	-
D0638_RS15555	15841..17316	-	1476	WP_149350365.1	primase C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cpsF	1..32648	32648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11371.98 Da
• Isoelectric Point: 9.5081

>T110077 WP_003573774.1 NZ_CP031786:c13164-12883 [Lacticaseibacillus paracasei]
MNKFVYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T110077 NZ_CP031786:c13164-12883 [Lacticaseibacillus paracasei]
ATGAATAAATTTGTTTATAAACCAGCCTTTGAACGTCAATTTAAAAAACATTACAAGACGATTTTAAAAGGGGGGCGCTA
TAAAAAAGAAGATTTTGAAGCTGTTTATCATAAACTATTACGAGATGAACCGTTAGATCCGCATTATAATGATCACGCTT
TGGTTAATCGTAAACCAGAGCGTGATTTACATATCAAGCCGGATTGGTTATTAATTTACAAATATGATGGTGAGTTTGTG
CGCTTTATCGACACGGGTTCGCATTCAGATCTTTTTAACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9217.44 Da
• Isoelectric Point: 5.1548

>AT110077 WP_016363783.1 NZ_CP031786:c13399-13157 [Lacticaseibacillus paracasei]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT110077 NZ_CP031786:c13399-13157 [Lacticaseibacillus paracasei]
ATGACTAGCCAAGTACATTTCAGAATGAGCGAAAAAGAAAAAAAGGACTTCGAGATTGTCCTAAGTCGTGTGGGGTTAAC
TCCCGGGGAAGCTTTTCGTATCTTTGCCAAGAAATCCATTGAAGCCGGAGGAATTCCCTTTGAGGTCTCTCAGCCAAACA
CACGTTTAAAAAAGGCTATTAGGAGTCAAGATTACGTTGAATTTAACGATCCTGAGGAAGGGCTGGATTGGTTAAATGAA
TAA

Structures

Toxin

• AlphaFold DB: A0A243Q0H7