Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1318254..1318818 Replicon chromosome
Accession NZ_CP031785
Organism Lacticaseibacillus paracasei strain 10266

Toxin (Protein)


Gene name yafQ Uniprot ID A0A510WIJ9
Locus tag D0638_RS07040 Protein ID WP_016363448.1
Coordinates 1318516..1318818 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag D0638_RS07035 Protein ID WP_003570051.1
Coordinates 1318254..1318523 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0638_RS07005 1313259..1314146 - 888 WP_003570041.1 ABC transporter ATP-binding protein -
D0638_RS07010 1314343..1315569 - 1227 WP_003570043.1 hypothetical protein -
D0638_RS07015 1315767..1316171 + 405 WP_003570045.1 MerR family transcriptional regulator -
D0638_RS07020 1316164..1316490 + 327 WP_003584272.1 YrdB family protein -
D0638_RS07025 1316553..1317182 + 630 WP_003570047.1 DUF2399 domain-containing protein -
D0638_RS07030 1317227..1317880 + 654 WP_003570049.1 GNAT family N-acetyltransferase -
D0638_RS07035 1318254..1318523 + 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
D0638_RS07040 1318516..1318818 + 303 WP_016363448.1 type II toxin-antitoxin system YafQ family toxin Toxin
D0638_RS15760 1319157..1319300 + 144 WP_162259784.1 hypothetical protein -
D0638_RS07045 1319418..1320110 + 693 WP_003570055.1 hypothetical protein -
D0638_RS07050 1320346..1322523 + 2178 WP_003570057.1 RNA-binding transcriptional accessory protein -
D0638_RS07055 1322948..1323505 - 558 WP_003584278.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-97)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11510.33 Da        Isoelectric Point: 9.8881

>T110075 WP_016363448.1 NZ_CP031785:1318516-1318818 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELYVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLVK

Download         Length: 303 bp

>T110075 NZ_CP031785:1318516-1318818 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCCGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCCT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTATATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTGAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGTTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT110075 WP_003570051.1 NZ_CP031785:1318254-1318523 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT110075 NZ_CP031785:1318254-1318523 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAGGAAACTCGCTTGAATATTCGTGTTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A510WIJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References