Detailed information of TA system TA110029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1116247..1116670
• Replicon: chromosome
• Accession: NZ_CP031779
• Organism: Staphylococcus aureus strain CFBR-105

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U7EW16
• Locus tag: D1F65_RS05625
• Protein ID: WP_001065092.1
• Coordinates: 1116247..1116498 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0E0VQM8
• Locus tag: D1F65_RS05630
• Protein ID: WP_000028422.1
• Coordinates: 1116488..1116670 (+)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1F65_RS05585	1111804..1112724	+	921	WP_000138475.1	recombinase RecT	-
D1F65_RS05590	1112805..1113422	+	618	WP_071890040.1	MBL fold metallo-hydrolase	-
D1F65_RS05595	1113423..1113893	+	471	WP_025380777.1	single-stranded DNA-binding protein	-
D1F65_RS05600	1113923..1114810	+	888	WP_000148306.1	DnaD domain protein	-
D1F65_RS05605	1114817..1115035	+	219	WP_000338528.1	hypothetical protein	-
D1F65_RS05610	1115044..1115448	+	405	WP_000401971.1	RusA family crossover junction endodeoxyribonuclease	-
D1F65_RS05615	1115461..1115829	+	369	WP_075345438.1	hypothetical protein	-
D1F65_RS05620	1115833..1116075	+	243	WP_000131366.1	hypothetical protein	-
D1F65_RS14315	1116087..1116254	+	168	WP_001624242.1	hypothetical protein	-
D1F65_RS05625	1116247..1116498	+	252	WP_001065092.1	DUF1024 family protein	Toxin
D1F65_RS05630	1116488..1116670	+	183	WP_000028422.1	hypothetical protein	Antitoxin
D1F65_RS05635	1116663..1117205	+	543	WP_000185659.1	dUTP pyrophosphatase	-
D1F65_RS05640	1117242..1117448	+	207	WP_000195803.1	DUF1381 domain-containing protein	-
D1F65_RS05645	1117445..1117828	+	384	WP_000592229.1	hypothetical protein	-
D1F65_RS05650	1117828..1118001	+	174	WP_000595244.1	transcriptional activator RinB	-
D1F65_RS05655	1118002..1118148	+	147	WP_000990001.1	hypothetical protein	-
D1F65_RS05660	1118172..1118594	+	423	WP_000162696.1	RinA family phage transcriptional activator	-
D1F65_RS05665	1118781..1119275	+	495	WP_001038244.1	terminase small subunit	-
D1F65_RS05670	1119278..1120573	+	1296	WP_000273011.1	PBSX family phage terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1103256..1146096	42840

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9375.25 Da
• Isoelectric Point: 3.8567

>T110029 WP_001065092.1 NZ_CP031779:1116247-1116498 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQYSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEH

Nucleotide

• Length: 252 bp

>T110029 NZ_CP031779:1116247-1116498 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTTTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAATATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6741.63 Da
• Isoelectric Point: 4.2658

>AT110029 WP_000028422.1 NZ_CP031779:1116488-1116670 [Staphylococcus aureus]
MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLIDFVVKPIHLIKEEQEND

Nucleotide

• Length: 183 bp

>AT110029 NZ_CP031779:1116488-1116670 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Structures

Toxin

• AlphaFold DB: A0A7U7EW16

Antitoxin

• AlphaFold DB: A0A0E0VQM8