Detailed information of TA system TA109936

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 100254..100507
• Replicon: plasmid pKPC2_020003
• Accession: NZ_CP031720
• Organism: Klebsiella pneumoniae strain SCKP020003

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CAY64_RS00960
• Protein ID: WP_001312851.1
• Coordinates: 100254..100403 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	100448..100507 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY64_RS00910	95613..96028	-	416	Protein_117	IS1 family transposase	-
CAY64_RS00925	96277..96678	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
CAY64_RS28920	96611..96868	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
CAY64_RS00930	96961..97614	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
CAY64_RS00940	98553..99410	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
CAY64_RS00945	99403..99477	-	75	WP_001375168.1	RepA leader peptide Tap	-
CAY64_RS00955	99722..99970	-	249	WP_000083837.1	replication regulatory protein RepA	-
CAY64_RS00960	100254..100403	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	100448..100507	+	60	NuclAT_1	-	Antitoxin
-	100448..100507	+	60	NuclAT_1	-	Antitoxin
-	100448..100507	+	60	NuclAT_1	-	Antitoxin
-	100448..100507	+	60	NuclAT_1	-	Antitoxin
CAY64_RS00970	100708..100938	-	231	WP_001736714.1	hypothetical protein	-
CAY64_RS00975	101102..101701	-	600	WP_032083981.1	hypothetical protein	-
CAY64_RS00980	102087..102287	-	201	WP_015059022.1	hypothetical protein	-
CAY64_RS00985	102419..102979	-	561	WP_000139328.1	fertility inhibition protein FinO	-
CAY64_RS00990	103034..103780	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
CAY64_RS00995	103800..103961	-	162	Protein_130	DNA helicase	-
CAY64_RS01000	104025..104729	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CAY64_RS01005	104782..104847	+	66	Protein_132	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2	-	1..154957	154957

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T109936 WP_001312851.1 NZ_CP031720:c100403-100254 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T109936 NZ_CP031720:c100403-100254 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT109936 NZ_CP031720:100448-100507 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin