Detailed information of TA system TA109932

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59341..59767
• Replicon: plasmid pKPC2_020003
• Accession: NZ_CP031720
• Organism: Klebsiella pneumoniae strain SCKP020003

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CAY64_RS28910
• Protein ID: WP_001312861.1
• Coordinates: 59341..59499 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	59543..59767 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY64_RS00590	54396..54623	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
CAY64_RS00595	54717..55403	-	687	WP_015059009.1	PAS domain-containing protein	-
CAY64_RS00600	55594..55977	-	384	WP_000124981.1	relaxosome protein TraM	-
CAY64_RS00605	56254..56901	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
CAY64_RS00610	57198..58019	-	822	WP_001234445.1	DUF945 domain-containing protein	-
CAY64_RS00615	58130..58426	-	297	WP_001272251.1	hypothetical protein	-
CAY64_RS30350	58726..59103	+	378	Protein_71	hypothetical protein	-
CAY64_RS28910	59341..59499	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	59543..59767	-	225	NuclAT_0	-	Antitoxin
-	59543..59767	-	225	NuclAT_0	-	Antitoxin
-	59543..59767	-	225	NuclAT_0	-	Antitoxin
-	59543..59767	-	225	NuclAT_0	-	Antitoxin
CAY64_RS00640	59779..60498	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
CAY64_RS00645	60495..60929	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CAY64_RS00650	60998..63021	-	2024	Protein_75	ParB/RepB/Spo0J family partition protein	-
CAY64_RS00655	63082..63315	-	234	WP_000006003.1	DUF905 family protein	-
CAY64_RS00660	63373..63900	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2	-	1..154957	154957

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T109932 WP_001312861.1 NZ_CP031720:c59499-59341 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T109932 NZ_CP031720:c59499-59341 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT109932 NZ_CP031720:c59767-59543 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin