Detailed information of TA system TA109923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2024..2675
• Replicon: plasmid p3_010060
• Accession: NZ_CP031711
• Organism: Acinetobacter wuhouensis strain WCHA60

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9BC17
• Locus tag: BEN71_RS00430
• Protein ID: WP_004967307.1
• Coordinates: 2319..2675 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BEN71_RS00425
• Protein ID: WP_001140620.1
• Coordinates: 2024..2326 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN71_RS00415	11..1198	-	1188	WP_065996278.1	Tet(A)/Tet(B)/Tet(C) family tetracycline efflux MFS transporter	-
BEN71_RS00420	1264..1905	+	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
BEN71_RS00425	2024..2326	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
BEN71_RS00430	2319..2675	-	357	WP_004967307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BEN71_RS00440	2896..3732	-	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
BEN71_RS00445	3732..4535	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
BEN71_RS00450	4705..5520	-	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39) / aph(6)-Id / aph(3'')-Ib / sul2	-	1..12086	12086
	-	inside	IScluster/Tn	tet(39) / aph(6)-Id / aph(3'')-Ib / sul2	-	11..6874	6863

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13601.62 Da
• Isoelectric Point: 6.4648

>T109923 WP_004967307.1 NZ_CP031711:c2675-2319 [Acinetobacter wuhouensis]
MWIVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIADKGSKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T109923 NZ_CP031711:c2675-2319 [Acinetobacter wuhouensis]
ATGTGGATAGTCATTACGACAGATCTATTTAATGAATGGCTTGAACAGCAAGACGAATCAACACAAGAGAAGGTTCTGGC
TGCCCTAGTTGTTCTACAGCAGCAAGGACCAAGTTTAGGCCGCCCTTTAGTAGATACTGTCTATGACTCTAAATTTACCA
ACATGAAAGAGCTTCGTGTTCAACATCGAGGTAAACCACTGAGAGCTTTCTTCGCATTTGATCCTTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCAGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACCACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT109923 WP_001140620.1 NZ_CP031711:c2326-2024 [Acinetobacter wuhouensis]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT109923 NZ_CP031711:c2326-2024 [Acinetobacter wuhouensis]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCAGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTTGAAATCTCTCAAAAGGAACTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGCGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7T4TW67