Detailed information of TA system TA109921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 20040..20625
• Replicon: plasmid p2_010060
• Accession: NZ_CP031710
• Organism: Acinetobacter wuhouensis strain WCHA60

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: BEN71_RS00340
• Protein ID: WP_000897307.1
• Coordinates: 20040..20360 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BEN71_RS00345
• Protein ID: WP_000369781.1
• Coordinates: 20353..20625 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN71_RS00320	15279..17000	+	1722	WP_161553503.1	MobA/MobL family protein	-
BEN71_RS00325	17639..18583	+	945	WP_068912167.1	replication initiation protein RepM	-
BEN71_RS00330	18587..19141	+	555	WP_068975321.1	helix-turn-helix domain-containing protein	-
BEN71_RS19160	19154..19297	+	144	WP_166135746.1	hypothetical protein	-
BEN71_RS00335	19481..19900	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
BEN71_RS00340	20040..20360	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BEN71_RS00345	20353..20625	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
BEN71_RS00350	21118..22593	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
BEN71_RS00355	22649..23533	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
BEN71_RS00360	23723..24334	-	612	WP_015060246.1	recombinase family protein	-
BEN71_RS00365	24531..25256	-	726	WP_068975322.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E)	-	1..30787	30787
	-	inside	IScluster/Tn	msr(E) / mph(E)	-	21118..28026	6908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T109921 WP_000897307.1 NZ_CP031710:20040-20360 [Acinetobacter wuhouensis]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T109921 NZ_CP031710:20040-20360 [Acinetobacter wuhouensis]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT109921 WP_000369781.1 NZ_CP031710:20353-20625 [Acinetobacter wuhouensis]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT109921 NZ_CP031710:20353-20625 [Acinetobacter wuhouensis]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3