Detailed information of TA system TA109920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 8557..9142
• Replicon: plasmid p2_010060
• Accession: NZ_CP031710
• Organism: Acinetobacter wuhouensis strain WCHA60

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A385C123
• Locus tag: BEN71_RS00270
• Protein ID: WP_058971762.1
• Coordinates: 8557..8877 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BEN71_RS00275
• Protein ID: WP_000369781.1
• Coordinates: 8870..9142 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN71_RS00230	4015..4620	-	606	WP_152033024.1	hypothetical protein	-
BEN71_RS00235	4626..5003	-	378	WP_068975486.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
BEN71_RS00240	4981..5448	-	468	WP_068975487.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
BEN71_RS00245	5463..5837	-	375	WP_068975488.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
BEN71_RS00255	6436..6750	-	315	WP_058971764.1	hypothetical protein	-
BEN71_RS00265	8130..8390	-	261	WP_068976015.1	hypothetical protein	-
BEN71_RS00270	8557..8877	+	321	WP_058971762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BEN71_RS00275	8870..9142	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
BEN71_RS00280	9256..9918	+	663	WP_068976016.1	SH3 domain-containing protein	-
BEN71_RS00285	9968..11100	+	1133	Protein_14	IS3-like element ISAba14 family transposase	-
BEN71_RS00290	11324..11791	-	468	WP_068976124.1	hypothetical protein	-
BEN71_RS00295	11788..12123	-	336	WP_068976125.1	STAS-like domain-containing protein	-
BEN71_RS00300	12116..12607	-	492	WP_068976126.1	hypothetical protein	-
BEN71_RS00305	12701..13833	-	1133	Protein_18	IS3-like element ISAba14 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E)	-	1..30787	30787
	-	inside	IScluster/Tn	-	-	6873..13845	6972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12207.98 Da
• Isoelectric Point: 6.9800

>T109920 WP_058971762.1 NZ_CP031710:8557-8877 [Acinetobacter wuhouensis]
MLFIETSIFTKQIKDLVSDEEYRQLQEDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T109920 NZ_CP031710:8557-8877 [Acinetobacter wuhouensis]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGGA
AGATCTTTTGGTACAGCCTGATAGAGGTGATCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGAAAAAGTGGTGGCATTCGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATATTTTTCTTAGTGGCTTAT
CCAAAATCAGTGAAAGATAATTTAACAGATAAGGAAACAGCCATTCTGCGCCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT109920 WP_000369781.1 NZ_CP031710:8870-9142 [Acinetobacter wuhouensis]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT109920 NZ_CP031710:8870-9142 [Acinetobacter wuhouensis]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
TCGAGTTACAGAACTCGAACTACCAGATATTAAAGAAGTACGTGAAAAAACAGGCTTGAGCCAAAATGAGTTTGCTGCAC
GTCTGCATATTAGTCCTCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCATCCAAGTCTTATTTAA

Structures

Toxin

• AlphaFold DB: A0A385C123