Detailed information of TA system TA109912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4172035..4172834
• Replicon: chromosome
• Accession: NZ_CP031706
• Organism: Escherichia coli strain R1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: D0T88_RS20085
• Protein ID: WP_000347267.1
• Coordinates: 4172370..4172834 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: D0T88_RS20080
• Protein ID: WP_001307405.1
• Coordinates: 4172035..4172370 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0T88_RS20065	4167820..4168590	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
D0T88_RS20070	4168606..4169940	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
D0T88_RS20075	4170315..4171886	+	1572	WP_001273741.1	galactarate dehydratase	-
D0T88_RS20080	4172035..4172370	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
D0T88_RS20085	4172370..4172834	+	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
D0T88_RS20090	4172889..4173698	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
D0T88_RS20095	4173947..4175227	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
D0T88_RS20100	4175250..4175723	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
D0T88_RS20105	4175734..4176513	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
D0T88_RS20110	4176503..4177381	+	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
D0T88_RS20115	4177399..4177833	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4160100..4172834	12734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T109912 WP_000347267.1 NZ_CP031706:4172370-4172834 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T109912 NZ_CP031706:4172370-4172834 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT109912 WP_001307405.1 NZ_CP031706:4172035-4172370 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT109912 NZ_CP031706:4172035-4172370 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7