Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2814603..2815105 Replicon chromosome
Accession NZ_CP031706
Organism Escherichia coli strain R1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag D0T88_RS13770 Protein ID WP_000767829.1
Coordinates 2814603..2814857 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag D0T88_RS13775 Protein ID WP_001259255.1
Coordinates 2814854..2815105 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0T88_RS13740 2809618..2809845 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
D0T88_RS13745 2809859..2810917 - 1059 WP_000492346.1 transport protein YeeE -
D0T88_RS13750 2811096..2812454 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
D0T88_RS13755 2812444..2812506 - 63 WP_010723108.1 membrane protein YoeI -
D0T88_RS13760 2812721..2813650 - 930 WP_000803351.1 LysR family transcriptional regulator -
D0T88_RS13765 2813696..2814520 - 825 WP_000754758.1 SDR family oxidoreductase -
D0T88_RS13770 2814603..2814857 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
D0T88_RS13775 2814854..2815105 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
D0T88_RS13780 2815388..2815438 + 51 WP_001364200.1 his operon leader peptide -
D0T88_RS13785 2815584..2816483 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
D0T88_RS13790 2816489..2817793 + 1305 WP_001542955.1 histidinol dehydrogenase -
D0T88_RS13795 2817790..2818860 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
D0T88_RS13800 2818860..2819927 + 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T109908 WP_000767829.1 NZ_CP031706:c2814857-2814603 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T109908 NZ_CP031706:c2814857-2814603 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT109908 WP_001259255.1 NZ_CP031706:c2815105-2814854 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT109908 NZ_CP031706:c2815105-2814854 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References