Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 868222..868763 Replicon chromosome
Accession NZ_CP031706
Organism Escherichia coli strain R1

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag D0T88_RS04070 Protein ID WP_000615982.1
Coordinates 868222..868500 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag D0T88_RS04075 Protein ID WP_000729704.1
Coordinates 868503..868763 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0T88_RS04055 865804..866382 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
D0T88_RS04060 866588..867355 + 768 WP_000333380.1 class II glutamine amidotransferase -
D0T88_RS04065 867326..868066 - 741 WP_001225679.1 murein L,D-transpeptidase -
D0T88_RS04070 868222..868500 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
D0T88_RS04075 868503..868763 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
D0T88_RS04080 868949..869722 + 774 WP_161396232.1 C40 family peptidase -
D0T88_RS04085 869779..870135 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
D0T88_RS04090 870128..870406 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
D0T88_RS04095 870511..872250 - 1740 WP_001298878.1 flagellar biosynthesis protein FlhA -
D0T88_RS04100 872195..872980 + 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T109893 WP_000615982.1 NZ_CP031706:c868500-868222 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T109893 NZ_CP031706:c868500-868222 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT109893 WP_000729704.1 NZ_CP031706:c868763-868503 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT109893 NZ_CP031706:c868763-868503 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References