Detailed information of TA system TA109870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 220268..221013
• Replicon: chromosome
• Accession: NZ_CP031695
• Organism: Erwinia billingiae strain TH88

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D0N50_RS00990
• Protein ID: WP_151036322.1
• Coordinates: 220268..220579 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D0N50_RS00995
• Protein ID: WP_151036323.1
• Coordinates: 220576..221013 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0N50_RS00970	216394..217326	+	933	WP_013204359.1	ABC transporter permease subunit	-
D0N50_RS00975	217323..218222	+	900	WP_151036319.1	ABC transporter permease subunit	-
D0N50_RS00980	218227..219282	+	1056	WP_151036320.1	P1 family peptidase	-
D0N50_RS00985	219350..220171	+	822	WP_151036321.1	M55 family metallopeptidase	-
D0N50_RS00990	220268..220579	+	312	WP_151036322.1	type II toxin-antitoxin system HigB family toxin	Toxin
D0N50_RS00995	220576..221013	+	438	WP_151036323.1	helix-turn-helix domain-containing protein	Antitoxin
D0N50_RS01000	221097..221336	+	240	WP_053141357.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
D0N50_RS01005	221345..221776	+	432	WP_151038647.1	type II toxin-antitoxin system VapC family toxin	-
D0N50_RS01010	221773..223272	-	1500	WP_151036324.1	serine hydrolase	-
D0N50_RS01015	223269..224009	-	741	WP_053141361.1	ABC transporter ATP-binding protein	-
D0N50_RS01020	224012..224842	-	831	WP_151036325.1	ABC transporter ATP-binding protein	-
D0N50_RS01025	224843..225742	-	900	WP_013204352.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (89-140)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12566.39 Da
• Isoelectric Point: 10.1185

>T109870 WP_151036322.1 NZ_CP031695:220268-220579 [Erwinia billingiae]
MRVISRKPFEQAARSYPNDAGALDDTYRVLHTEKFLTPDLLKARFRSLDRFKYRDKWWVIDVGGNNLRIITYIKFETQNL
FIKHIVPHKEYDRLTEYYRRNPE

Nucleotide

• Length: 312 bp

>T109870 NZ_CP031695:220268-220579 [Erwinia billingiae]
ATGCGCGTTATTTCAAGGAAGCCATTTGAGCAAGCCGCCCGATCTTATCCAAATGATGCAGGAGCTTTGGATGATACTTA
TCGGGTTCTTCATACCGAAAAATTTTTGACACCTGATCTGTTAAAAGCCCGTTTTCGTTCACTGGATCGTTTTAAATATC
GGGACAAATGGTGGGTTATCGATGTGGGTGGAAATAATCTCAGGATTATTACCTACATAAAATTTGAGACGCAGAACCTC
TTTATCAAACATATCGTGCCCCATAAAGAGTATGACAGGTTGACTGAGTATTACCGGAGGAACCCAGAATGA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16289.61 Da
• Isoelectric Point: 5.5403

>AT109870 WP_151036323.1 NZ_CP031695:220576-221013 [Erwinia billingiae]
MRAAHTVKYQHVIHATEILVNAVPFLGANPEQADYKEALKLVEYLLEHNDDSPLIDLLATRIARYEDTAAEFTDFNARIA
AIPQELAMLRTLMDQYGLNQSSFQQEIGSRSLVSMIMNGQRALTLDHMRALSARFNIPISAFING

Nucleotide

• Length: 438 bp

>AT109870 NZ_CP031695:220576-221013 [Erwinia billingiae]
ATGAGAGCCGCTCACACCGTTAAATATCAACACGTAATCCATGCAACTGAGATCCTGGTGAATGCAGTGCCTTTTCTGGG
GGCGAATCCTGAGCAGGCCGATTATAAAGAAGCGCTAAAACTGGTTGAGTATTTACTTGAGCACAATGATGACAGCCCGT
TGATTGACCTGCTGGCGACAAGAATTGCACGCTATGAAGACACCGCGGCTGAGTTTACGGATTTTAACGCGCGTATCGCC
GCAATCCCCCAAGAATTAGCCATGCTCAGGACCTTAATGGACCAATATGGACTTAATCAAAGCAGCTTCCAGCAGGAGAT
AGGCAGCCGTTCTTTGGTGAGTATGATCATGAACGGACAGCGTGCGTTGACGCTGGATCATATGCGCGCGCTGTCAGCCC
GTTTCAATATTCCCATTTCGGCCTTCATTAATGGTTAA