Detailed information of TA system TA109861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1222023..1222662
• Replicon: chromosome
• Accession: NZ_CP031692
• Organism: Haemophilus influenzae strain P595-8370

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: CH556_RS06030
• Protein ID: WP_005650215.1
• Coordinates: 1222023..1222328 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CH556_RS06035
• Protein ID: WP_005650217.1
• Coordinates: 1222339..1222662 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH556_RS06010	1217464..1219503	-	2040	WP_042611248.1	excinuclease ABC subunit B	-
CH556_RS06020	1220107..1221075	-	969	WP_110426067.1	zinc transporter permease subunit ZevB	-
CH556_RS06025	1221078..1221698	-	621	WP_005650213.1	zinc transporter binding subunit ZevA	-
CH556_RS06030	1222023..1222328	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH556_RS06035	1222339..1222662	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
CH556_RS06040	1222825..1224495	+	1671	WP_048954581.1	energy-dependent translational throttle protein EttA	-
CH556_RS06045	1224557..1224898	+	342	WP_005666735.1	SirB2 family protein	-
CH556_RS06050	1224903..1226747	+	1845	WP_116945356.1	tRNA(Met) cytidine acetyltransferase TmcA	-
CH556_RS09185	1226744..1226917	+	174	WP_005657572.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T109861 WP_005650215.1 NZ_CP031692:1222023-1222328 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T109861 NZ_CP031692:1222023-1222328 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT109861 WP_005650217.1 NZ_CP031692:1222339-1222662 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT109861 NZ_CP031692:1222339-1222662 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACACTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70