Detailed information of TA system TA109853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1233329..1233968
• Replicon: chromosome
• Accession: NZ_CP031691
• Organism: Haemophilus influenzae strain P615-8618

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1B8PGE6
• Locus tag: CH576_RS06165
• Protein ID: WP_012054677.1
• Coordinates: 1233329..1233634 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CH576_RS06170
• Protein ID: WP_050848570.1
• Coordinates: 1233645..1233968 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH576_RS06145	1228758..1230797	-	2040	WP_005694308.1	excinuclease ABC subunit B	-
CH576_RS06155	1231412..1232380	-	969	WP_005650211.1	zinc transporter permease subunit ZevB	-
CH576_RS06160	1232383..1233003	-	621	WP_105894794.1	zinc transporter binding subunit ZevA	-
CH576_RS06165	1233329..1233634	+	306	WP_012054677.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH576_RS06170	1233645..1233968	+	324	WP_050848570.1	HigA family addiction module antidote protein	Antitoxin
CH576_RS06175	1234131..1235801	+	1671	WP_105894795.1	energy-dependent translational throttle protein EttA	-
CH576_RS06180	1235863..1236204	+	342	WP_005652996.1	SirB2 family protein	-
CH576_RS06185	1236209..1238053	+	1845	WP_105894799.1	tRNA(Met) cytidine acetyltransferase TmcA	-
CH576_RS09210	1238050..1238223	+	174	WP_162877173.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-67)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12180.80 Da
• Isoelectric Point: 8.3426

>T109853 WP_012054677.1 NZ_CP031691:1233329-1233634 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDAHSKIPSNLSKVLARKLDMISAAEDINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T109853 NZ_CP031691:1233329-1233634 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATGCTCATAGTAAGAT
TCCTTCTAATTTATCTAAAGTATTGGCAAGAAAATTGGATATGATAAGTGCTGCAGAAGACATCAATGATTTACGTTCTC
CTCCAGCTAATCACTTAGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTAAATAAACAATATCGCCTA
ATTTTTAAATATGAGAATAATGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12247.06 Da
• Isoelectric Point: 8.8251

>AT109853 WP_050848570.1 NZ_CP031691:1233645-1233968 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKINDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKAFDTTPEFWLNLQTRIDLWNL
EHNKRFQQSLANVKSALHRHGTSAFAM

Nucleotide

• Length: 324 bp

>AT109853 NZ_CP031691:1233645-1233968 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGCGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAATGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGCATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGAACCTT
GAACACAATAAACGTTTTCAACAAAGCCTTGCGAATGTTAAGTCCGCTCTACATCGGCATGGCACTTCTGCATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A1B8PGE6