Detailed information of TA system TA109847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1508733..1509372
• Replicon: chromosome
• Accession: NZ_CP031690
• Organism: Haemophilus influenzae strain P617-9224

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: CH578_RS07595
• Protein ID: WP_005650215.1
• Coordinates: 1509067..1509372 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CH578_RS07590
• Protein ID: WP_005650217.1
• Coordinates: 1508733..1509056 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH578_RS09265	1504352..1504525	-	174	WP_011272700.1	DUF5363 domain-containing protein	-
CH578_RS07575	1504522..1506492	-	1971	WP_011961904.1	tRNA(Met) cytidine acetyltransferase TmcA	-
CH578_RS07580	1506497..1506838	-	342	WP_005652996.1	SirB2 family protein	-
CH578_RS07585	1506900..1508570	-	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
CH578_RS07590	1508733..1509056	-	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
CH578_RS07595	1509067..1509372	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH578_RS07600	1509697..1510317	+	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
CH578_RS07605	1510320..1511288	+	969	WP_038441067.1	zinc transporter permease subunit ZevB	-
CH578_RS07615	1511904..1513937	+	2034	WP_070828296.1	excinuclease ABC subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T109847 WP_005650215.1 NZ_CP031690:c1509372-1509067 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T109847 NZ_CP031690:c1509372-1509067 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT109847 WP_005650217.1 NZ_CP031690:c1509056-1508733 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT109847 NZ_CP031690:c1509056-1508733 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70