Detailed information of TA system TA109840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1232018..1232657
• Replicon: chromosome
• Accession: NZ_CP031689
• Organism: Haemophilus influenzae strain P621-7028

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1B8PGE6
• Locus tag: CH582_RS06145
• Protein ID: WP_012054677.1
• Coordinates: 1232018..1232323 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CH582_RS06150
• Protein ID: WP_050848570.1
• Coordinates: 1232334..1232657 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH582_RS06125	1227447..1229486	-	2040	WP_005694308.1	excinuclease ABC subunit B	-
CH582_RS06135	1230101..1231069	-	969	WP_005650211.1	zinc transporter permease subunit ZevB	-
CH582_RS06140	1231072..1231692	-	621	WP_105894794.1	zinc transporter binding subunit ZevA	-
CH582_RS06145	1232018..1232323	+	306	WP_012054677.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH582_RS06150	1232334..1232657	+	324	WP_050848570.1	HigA family addiction module antidote protein	Antitoxin
CH582_RS06155	1232820..1234490	+	1671	WP_105894795.1	energy-dependent translational throttle protein EttA	-
CH582_RS06160	1234552..1234893	+	342	WP_005652996.1	SirB2 family protein	-
CH582_RS06165	1234898..1236742	+	1845	WP_105894799.1	tRNA(Met) cytidine acetyltransferase TmcA	-
CH582_RS09190	1236739..1236912	+	174	WP_162877173.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-67)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12180.80 Da
• Isoelectric Point: 8.3426

>T109840 WP_012054677.1 NZ_CP031689:1232018-1232323 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDAHSKIPSNLSKVLARKLDMISAAEDINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T109840 NZ_CP031689:1232018-1232323 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATGCTCATAGTAAGAT
TCCTTCTAATTTATCTAAAGTATTGGCAAGAAAATTGGATATGATAAGTGCTGCAGAAGACATCAATGATTTACGTTCTC
CTCCAGCTAATCACTTAGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTAAATAAACAATATCGCCTA
ATTTTTAAATATGAGAATAATGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12247.06 Da
• Isoelectric Point: 8.8251

>AT109840 WP_050848570.1 NZ_CP031689:1232334-1232657 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKINDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKAFDTTPEFWLNLQTRIDLWNL
EHNKRFQQSLANVKSALHRHGTSAFAM

Nucleotide

• Length: 324 bp

>AT109840 NZ_CP031689:1232334-1232657 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGCGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAATGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGCATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGAACCTT
GAACACAATAAACGTTTTCAACAAAGCCTTGCGAATGTTAAGTCCGCTCTACATCGGCATGGCACTTCTGCATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A1B8PGE6