Detailed information of TA system TA109826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1222013..1222652
• Replicon: chromosome
• Accession: NZ_CP031687
• Organism: Haemophilus influenzae strain P641-4342

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: CH602_RS06015
• Protein ID: WP_005650215.1
• Coordinates: 1222013..1222318 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CH602_RS06020
• Protein ID: WP_005650217.1
• Coordinates: 1222329..1222652 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH602_RS05995	1217443..1219482	-	2040	WP_042603038.1	excinuclease ABC subunit B	-
CH602_RS06005	1220097..1221065	-	969	WP_042593950.1	zinc transporter permease subunit ZevB	-
CH602_RS06010	1221068..1221688	-	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
CH602_RS06015	1222013..1222318	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH602_RS06020	1222329..1222652	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
CH602_RS06025	1222815..1224485	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
CH602_RS06030	1224547..1224888	+	342	WP_005652996.1	SirB2 family protein	-
CH602_RS06035	1224893..1226863	+	1971	WP_011961904.1	tRNA(Met) cytidine acetyltransferase TmcA	-
CH602_RS09220	1226860..1227033	+	174	WP_011272700.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T109826 WP_005650215.1 NZ_CP031687:1222013-1222318 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T109826 NZ_CP031687:1222013-1222318 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT109826 WP_005650217.1 NZ_CP031687:1222329-1222652 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT109826 NZ_CP031687:1222329-1222652 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70