Detailed information of TA system TA109767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1263234..1263873
• Replicon: chromosome
• Accession: NZ_CP031678
• Organism: Haemophilus influenzae strain P679-2791

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: CH639_RS06290
• Protein ID: WP_005650215.1
• Coordinates: 1263234..1263539 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CH639_RS06295
• Protein ID: WP_005650217.1
• Coordinates: 1263550..1263873 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH639_RS06270	1258663..1260702	-	2040	WP_038441065.1	excinuclease ABC subunit B	-
CH639_RS06280	1261318..1262286	-	969	WP_038441067.1	zinc transporter permease subunit ZevB	-
CH639_RS06285	1262289..1262909	-	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
CH639_RS06290	1263234..1263539	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH639_RS06295	1263550..1263873	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
CH639_RS06300	1264036..1265706	+	1671	WP_085000141.1	energy-dependent translational throttle protein EttA	-
CH639_RS06305	1265768..1266109	+	342	WP_050846956.1	SirB2 family protein	-
CH639_RS06310	1266114..1268084	+	1971	WP_085000143.1	tRNA(Met) cytidine acetyltransferase TmcA	-
CH639_RS09280	1268081..1268254	+	174	WP_011272700.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T109767 WP_005650215.1 NZ_CP031678:1263234-1263539 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T109767 NZ_CP031678:1263234-1263539 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT109767 WP_005650217.1 NZ_CP031678:1263550-1263873 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT109767 NZ_CP031678:1263550-1263873 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70